pathogenic bacteria, fish, DNA molecular, PCR
نویسندگان
چکیده
منابع مشابه
DNA extraction protocol for rapid PCR detection of pathogenic bacteria.
Twelve reagents were evaluated to develop a direct DNA extraction method suitable for PCR detection of foodborne bacterial pathogens. Many reagents exhibited strong PCR inhibition, requiring significant dilution of the extract with a corresponding reduction in sensitivity. Most reagents also exhibited much lower recovery of DNA from the gram-positive test organism (Listeria monocytogenes) than ...
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For the rapid identification and detection of Vibrio anguillarum, we have PCR amplification technique targeting gyrB region has been evaluated. We have designed twosets of PCR primers for the specific amplification of gyrB in V. anguillarum using single and nested PCR. PCRs specificity was demonstrated by successful amplicon from V. anguillarum DNA. The detection limit of the single PCRs and th...
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Virulence gene expression in pathogenic bacteria is modulated by environmental parameters. A key factor in this expression is temperature. Its effect on virulence gene expression in bacteria infecting warm-blooded hosts is well documented. Transcription of virulence genes in these bacteria is induced upon a shift from low environmental to a higher host temperature (37°C). Interestingly, host te...
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Chemotherapeutic agents have been developed for treating bacterial infections and have been widely used for cultured fish for the last 30 years in Japan. The extensive use of chemotherapeutants has resulted in an increase in the occurrence of drug resistance in fish-pathogenic bacteria and also in the bacterial flora of the intestinal tract of cultured fish. The kinds of chemotherapeutants used...
متن کاملAntibacterial Activity of Marine Sponge Extracts against Fish Pathogenic Bacteria
Secondary metabolites of the marine sponges Acanthella elongata, Axinella donnani, Callyspongia diffusa, Callyspongia subarmigera, and Echinodictyum gorgonoides were collected from fishing nets and their in vitro antibacterial properties against eight virulent marine fish pathogens were studied at incubation temperatures of 20°C and 30°C. Crude methanol extracts of the tested sponges showed spe...
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ژورنال
عنوان ژورنال: Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
سال: 2010
ISSN: 2406-9272,2089-5690
DOI: 10.15578/squalen.v5i2.49